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The two protein bands from forty kDa to 30 kDa ended up absent soon after 24 h incubation at forty five °C. For the rest of 23hoki the bands, several concentrations of all a few proteases retained protein bands similar to their initial molar masses (Figure 6a,b). The presence of bands at lower molecular weight in fish roe right before hydrolysis was unexpected. From the report by Ghelichi et al. [eight], insignificant peaks of a comparatively lower molecular pounds array (3–14 kDa) were being noticed in defatted carp roe homogenate, perhaps as a consequence of an autolytic system transpiring in fish roe and/or the defatting approach leading to the development of minimal-molecular-excess weight peptides.
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